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Srinagar : The CSIR–Indian Institute of Integrative Medicine (CSIR-IIIM), Srinagar Branch, has commenced a three-day hands-on training programme on quantitative real-time PCR (qRT-PCR), aimed at enhancing practical competencies in advanced molecular biology techniques among students, research scholars and laboratory professionals. The programme is designed to provide participants with comprehensive, bench-level proficiency in gene expression analysis, covering the entire workflow from sample preparation to data interpretation.
In his virtual address, Dr. Zabeer Ahmed, Director, CSIR-IIIM, highlighted the critical importance of qRT-PCR in contemporary molecular biology. He emphasised its indispensable role in precise transcript quantification, validation of high-throughput genomic data, and its applications in translational research and molecular diagnostics.
The intensive curriculum spans the complete experimental pipeline, beginning with high-quality RNA isolation and contamination control, followed by optimised reverse transcription strategies, primer design considerations, and selection of appropriate chemistries including SYBR Green and probe-based assays. Participants receive structured training in instrument setup, run execution, and robust data analysis methodologies. The programme places strong emphasis on practical skill development through extended wet lab sessions on RNA isolation, cDNA synthesis, and qRT-PCR, complemented by troubleshooting modules addressing common challenges such as low yields, non-specific amplification, efficiency variations, and inter-run inconsistencies. Good laboratory practices and quality control measures form an integral part of the training.
The programme is being organised at CSIR-IIIM Srinagar Branch by Dr. Nasheeman Ashraf, Senior Principal Scientist, and co-organised by Dr. Syed Reyazul Hassan, Senior Principal Scientist, and Dr. Phalesteen Sultan, Principal Technical Officer, under the patronage of Er. Abdul Rahim, Chief Scientist and Head, IIIM Srinagar Branch. Conducted under the CSIR-Integrated Skill Initiatives, the training is scheduled from February 9 to 11, 2026, and has attracted around 20 participants from various parts of the country.
